Venous Invasion in Cancer of the Stomach -With a Special Reference to Method of Histological Examination-

Kiyoshi Inada, Kuniyasu Shimokawa, Tsuneko Ikeda*, Yutaka Ozeki**, Hiromi Tanemura***

*Department of Laboratory Medicine, **First Department of Surgery and ***Second Department of Surgery, Gifu University School of Medicine

Venous invasion in cancer of the stomach was investigated in two series which were similar in several clinicopathological aspects, one (A) consisting of 235 surgical cases and the other (B) of 115 cases. For histological examination double staining with Victoria blue and hematoxylin-eosin was routinely used, supplemented with endothelial staining using factor Vlll-related antigen and basement membrane staining using polyclonal antibody to laminin. The incidence of venous invasion was 37.0% in series A, while its incidence was 48.3% when the 55 cases in which invasion was confined to the mucosa were excluded. It was 56.0% in series B, though the average number of tissue sections examined in B was double that in series A. The incidence of venous invasion increased proportionately to the depth of penetration of the cancer, the degree of lymphatic invasion and lymph node metastasis, though no significant relationship was found between the incidence of venous invasion and the histologic type of cancer. Histological examination of tumor tissues which were obtained from both central and peripheral parts, totaling at least four pieces, were preferable for detection of venous invasion. Double staining with Victoria blue and hematoxylin-eosin proved to be useful for detecting venous invasion in carcinomatous tissue, though endothelial and basement membrane markers have great specifity.

Key words
venous invasion in gastric cancer, Victoria blue-hematoxylin eosin stain, factor VIII related antigen stain, laminin stain

Reprint requests
Kiyoshi Inada Department of Laboratory Medicine, Gifu University, School of Medicine
40 Tsukasa-machi, Gifu, 500 JAPAN

Accepted
April 11, 1990

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