Changes of Adenosine 5´-triphosphate and Tissue Blood Flow in Ischemia and Reperfusion of Jaundiced Rat Liver

Kei Watanabe, Atsuyoshi Onitsuka*, Shoshi Senga, Tomoyuki Miyata, Tatsumi Iida, Masatomo Hayashi, Hajime Hirose

First Department of Surgery, Gifu University School of Medicine
*Kaizu Medical Association Hospital

This study was focused on the changes in adenosine 5´-triphosphage (ATP) levels and tissue blood flow in ischemia and reperfusion, in jaundiced rat liver. Bile ducts of male Wistar rats were ligated to induce jaundice. Rats were divided into five groups by the time (1, 2, 3 and 4 weeks) after bile duct ligation (BDL) (BDL-l W, 2 W, 3 W, 4 W, respectively) and a control (no ligation). Ischemia was induced by clamping the portal vein and hepatic artery for 15 minutes and 60 minutes. The ATP level in BDL-2, 3, 4 W (jaundiced) group was already lower than that in the control group before ischemia was induced and remained lower during reperfusion. No significant differences in the ATP level were found after 15 minutes of ischemia between the control and jaundiced groups. The rates of recovery of the ATP level after reperfusion preceded by 15 minutes of ischemia ranged from 70-100% among the various groups and no significant differences in the rates of recovery were seen between the control and jaundiced groups. The rates of recovery of tissue blood flow after reperfusion preceded by 15 minutes of ischemia ranged from 80-100%, and there were no significant differences in the rates of recovery between the control and jaundiced groups. The rate of recovery of the ATP level after reperfusion preceded by 60 minutes of ischemia in the BDL-4 W group was significantly lower than that in the control. The rate of recovery of tissue blood flow 30 minutes after reperfusion preceded by 60 minutes of ischemia in the BDL-4 W group was significantly lower than in the control. From these results, we concluded that there were no significant differences in the rates of recovery of the ATP level and tissue blood flow after reperfusion preceded by 15 minutes of ischemia between the control and jaundiced groups, but the rates of recovery of the ATP level and tissue blood flow after reperfusion preceded by 60 minutes of ischemia were significantly lower in the BDL-4 W group than those in the control.

Key words
obstructive jaundice, ischemia, changes of adenosine 5'-triphosphate, tissue blood flow

Reprint requests
Kei Watanabe First Department of Surgery, Gifu University School of Medicine
40 Tsukasa-machi, Gifu, 500 JAPAN

Accepted
June 17, 1992

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