A Study on Preoperative Detection of the Risk of Local Recurrence in Rectal Cancer with DNA Analysis of Biopsied Specimens -Collation of DNA Analysis Data on Cases of Local Recurrence-

Toshimasa Yatsuoka, Hidetaka Mochizuki, Kazuo Hase, Masahiko Koike, Eishu Nakamura, Shoetsu Tamakuma, Nariyoshi Shinomiya*, Sumiaki Tsuru*

First Department of Surgery, National Defense Medical College
*Department of Bacteriology, National Defense Medical College

Cellular DNA analysis was performed on curatively resected paraffin-embedded rectal cancer specimens by using flow cytometry. A DNA index (DI) of 1.28 or more was seen in only one (8.3%) of 12 patients without local recurrence, but in 7 (41.2%) of 17 patients with local recurrence (p<0.05). The DI of preoperatively biopsied fresh specimens showed a significant linear correlation with the DI of paraffin-embedded resected specimens. It was concluded that analysis of the cellular DNA content of preoperatively biopsied fresh specimens might be useful for the detection of a high risk of local recurrence.

Key words
cellular DNA analysis, DNA index of rectal cancer, preoperatively biopsied specimens, curatively resected paraffin-embedded specimens, local recurrence of rectal cancer

Reprint requests
Toshimasa Yatsuoka First Department of Surgery, National Defense Medical College
3-2 Namiki, Tokorozawa, 359 JAPAN

Accepted
September 9, 1992

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