Study of DNA Ploidy Pattern and DNA Index Using Flow Cytometry in Distant Metastasis of Gastric Cancer

Yukio Imanishi, Kazuyoshi Kurooka, Hiromi Yamada, Yoshinori Fujii, Suguru Sato, Kiyotaka Okuno, Akira Tanaka, Katsuhisa Shindo, Masayuki Yasutomi

First Department of Surgery, Kinki University School of Medicine

The correlation of DNA ploidy pattern and DNA index (DI) with distant metastasis in advanced gastric cancer was analyzed using flow cytometry in 158 patients who underwent surgery in our institute from 1981 to 1988. The frequency of DNA aneuploidy was 53.7% among 41 cases of peritoneal dissemination, 80.6% among 36 cases of liver metastasis, 74.1% among 27 cases of both, and 46.3% among 54 non-metastati cases. The DI in all cases of peritoneal dissemination or non-metastasis was from 1.0 to 1.6, whereas the DI in 27.8% of cases of liver metastasis and 33.3% of cases of peritoneal dissemination and liver matastasis was more than 1.7. In terms of histopathological type, 68.3% of cases of peritoneal dissemination originated from undifferentiated adenocarcinoma, whereas 77.8% of cases of liver metastasis originated from differentiated adenocarcinoma. In conclusion, we found that not only histological type but also DNA ploidy pattern and DI were significantly related to distant metastasis in gastric cancer.

Key words
flow cytometry, DNA ploidy pattern, DNA index, distant metastasis of gastric cancer

Reprint requests
Yukio Imanishi First Department of Surgery, Kinki University School of Medicine
377-2 Ohnohigashi, Osaka-sayama City, 589 JAPAN

Accepted
June 14, 1993

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