Role of Motility Factor Released by SW1990 Pancreatic Cancer Cell in Invasion and Metastasis

Tetsuji Sawada, Yong-Suk Chung, Toyoshi Sogabe, Nobuya Yamada, Yuichi Arimoto, Masakazu Yashiro, Yoshito Yamashita, Satoshi Takatsuka, Masahiro Okuno, Michio Sowa

The First Department of Surgery, Osaka City University, Medical School

In this study, two pancreatic cancer cell lines, SW1990 and PANC-1, were examined for the relationship between cellular motility and metastatic potential in the intrasplenic injection model, and the role of the motility factor released by SW1990 cells in invasion and metastasis was investigated. When the in vitro invasion and motility of SW1990 and PANC-1 cells were measured by the MTT method using a Trans well chamber, the percentages of cell invasion were 22.6% and.7.3% respectively, and those of motility were 33.3% and 13.6%. The high motility of SW1990 was correlated with its in vivo metastatic potential. As the motility of PANC-1 cells could be increased 2-3 times by treatment with SW1990 serum free spent medium in a dose dependent fashion, SWl990 cells may have the ability to produce motility factor. Incubation of PANC-1 cells with SW1990 medium resulted in the formation of thin processes and a fibroblast-like morphology, and also induced micro-metastasis in liver by splenic injection. These findings suggested that this motility factor may play an important role in panceratic cancer invasion and metastasis.

Key words
cellular motility, motility factor, pancreatic cancer, invasion and metastasis

Reprint requests
Tetsuji Sawada The First Department of Surgery, Osaka, City University, Medical School
1-5-7 Asahimachi, Abeno-ku, Osaka, 545 JAPAN

Accepted
July 6, 1994

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