Effects of Intraperitoneal Cisplatin Injection on the Intestinal Anastomosis in Rats

Kazuto Sasaya, Yasushi Kaneko, Masakazu Iwamoto

Department of Surgery, Daisan Hospital, The Jikei University School of Medicine

We studied experimentally the effects of cisplatin (cisdichlorodiamine platinum: CDDP) on intestinal anastomosis in the Wistar male rat. We performed intestinal anastomosis under the ether anesthesia and fixed a thin sponge at the anastomotic site; at the same time cisplatin (3 mg/kg body weight) was injected intraperitoneally or intravenously. One week after the operation the anastomotic site was resected. We measured the hydroxyproline content in the sponge, carried out microscopic examination, and measured the bursting pressure. The mean hydroxyproline levels of the intraperitoneal injection group, the intra. venous injection group, and the control group were 0.29 mg/g, 1.61 mg/g, and 2.02 mg/g, respectively. We recognized a statistically significant difference in each group. Upon microscopic examination, the scores of granulation and collagen formation in the intraperitoneal injection group were decreased significantly. The bursting pressures in the intraperitoneal injection group were decreased significantly (the means of the intraperitoneal injection group, the intravenous injection group, and the control group were 145 mmHg, 205 mmHg, and 216 mmHg, respectively). Hyperbaric oxygen therapy was not significantly effective for impaired anastomotic healing by intraperitoneal cisplatin injection. One week after the anastomotic operation, when we injected cisplatin into the peritoneal cavity, impairment of the anastomotic healing was not seen.

Key words
wound healing of intestinal anastomosis, intraperitoneal chemotherapy, hyperbaric xoygen therapy, hydroxyproline content in sponge, bursting pressure of digestive canal

Reprint requests
Kazuto Sasaya Department of Surgery, Disan Hospital, Jikei University School of Medicine
4-11-1 Izumihoncho, Komae-shi, Tokyo, 201 JAPAN

Accepted
July 5, 1995

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