Evaluation of DNA and PCNA by Two Color Flow Cytometric Analysis in Colorectal Cancers

Hiroaki Yoshitake

Second Department of Surgery, School of Medicine, Fukuoka University

Two color flow cytometric analysis of DNA and a labeling rate of proliferating cell nuclear antigen (PCNA LR) was performed on cancer cells of 100 cases with colorectal carcinoma in relation to clinicopathological factors. DNA analysis demonstrated a diploid pattern in 33 cases and aneuploid in 67 cases. The mean of PCNA LR was significantly higher in cases with aneuploid pattern than in those with diploid pattern. Regarding as lymph node metastasis, IFN γ and advanced Dukes classification were more frequent in cases with high PCNA LR and aneuploid pattern than in those with low PCNA LR and diploid DNA (p<0.01, p<0.01, p<0.05), respectively. The survival rate was significantly lower in cases with high PCNA LR and aneuploid pattern than in those with low PCNA LR and diploid pattern. These results suggest that DNA-PCNA dual fluorescence analysis by flow cytometry might give effective parameter on evaluation of malignant potency of the colorectal carcinoma.

Key words
flow cytometry, DNA, PCNA, colorectal cancer

Reprint requests
Hiroaki Yoshitake The Second Department of Surgery, School of Medicine, Fukuoka University
Fukuoka, 814-80 JAPAN

Accepted
May 8, 1996

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