Effect of Trypsin and Taurocholate on Electrophysiological Characteristics of Isolated Rat Esophageal Mucosa

Akihiro Iwatsuki, Iwao Sasaki, Yasuhiko Kamiyama, Hiroo Naito, Yuji Funayama, Seiki Matsuno

First Department of Surgery, Tohoku University School of Medicine

Using Sprague-Dawley rats, the effect of trypsin and taurocholate on the electrophysiological characteristics of isolated esophageal mucosa was studied. After administration of trypsin, both the esophageal transepitherial potential difference (PD) and short circuit current (Isc) were increased and the esophageal transepitherial resistance (Rt) was significantly reduced. On the otherhand, there were no changes in these parameters after administration of taurocholate (TCA). Increased Isc after administration of trypsin was significantly reduced by amiloride administration. These results suggest that active ion transport in the esophageal mucosal cells is impaired by trypsin, but not bile acid in alkaline state. Compared to bile, pancreatic juice has more important role in the pathogenesis of reflux esophagitis after total gastrectomy.

Key words
alkaline reflux esophagitis, trypsin, bile acid, total gastrectomy

Reprint requests
Iwao Sasaki First Department of Surgery, Tohoku University School of Medicine
1-1 Seiryocho, Aobaku, Sendai, 980-8574 JAPAN

Accepted
February 12, 1998

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