Experimental Study on Necrosis and Regeneration of Ischemic and Non-ischemic Lobes after Transient Partial Ischemia of the Liver

Tatsuru Akashi, Yoshihiro Asanuma, Tsutomu Sato, Hiroshi Nanjo*, Kenji Koyama

First Department of Surgery, Akita University School of Medicine
*Second Department of Pathology, Akita University School of Medicine

Histological studies on hepatic necrosis and regeneration after transient ischemia of 70% of the liver by both inflow and outflow occlusion were carried out in rats. In the ischemic lobe massive hepatic necrosis was observed especially around the central vein just after ischemia, and the area was more, wide-spread with longer duration of hepatic ischemia. Even in the case of 120-min ischemia, the necrotic area disappeared by the 14th day after revascularization. The PCNA labeling index of hepatocytes showed the highest values on the 2nd day in both ischemic and non-ischemic lobes. With respect to survival, all rats with 90-min ischemia survived, while the survival rate of those with 120-min ischemia was 89%. In rats with of 90- and 120-min ischemia, with permanent inflow occlusion of the remnant lobe (30%) after transient ischemia of 70%, the survival rates decreased to 80% and 33%, respectively. Therefore, in case of more than 90-min ischemia of 70% of the liver, it is mandatory for the non-ischemic lobe to function properly during the period, so that the ischemic lobe recovers and the individual survives.

Key words
transient partial ischemia of the liver, hepatic necrosis, hepatic regeneration, PCNA

Reprint requests
Tatsuru Akashi First Department of Surgery, Akita University School of Medicine
1-1-1 Hondo, Akita, 010-8543 JAPAN

Accepted
May 19, 1998

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