Intraoperative Sectetin Test for Estimation of Curability of Gastrinoma Resection -Rapid Radioimmunoassay of Gastrin-

Yasuaki Hattori, Masayuki Imamura, Takayoshi Tobe

First Department of Surgery, Faculty of Medicine, Kyoto University

We have developed a rapid assay of gastrin by which immunoreactive gastrin (IRG) can be measured within 1.5 hours by changing the incubation condition of anti-gastrin antibody. The sensitivity of this method was 20 pg/ml, and the precision was satisfactory; 4.8±2.4% in the intraassay coefficient of variation, 3.8±1.0% in the interassay coefficient of variation. The results of the rapid assay were correlated with the conventional method: the regression equation was y=-26.97 +1.052x, and correlation coefficient was 0.994. This rapid gastrin assay was used in the Intraoperative Secretin test (IOS test) for three patients with Zollinger-Ellison syndrome. The curability of resection for gastrinoma (s) in these patients could be confirmed intraoperatively with the IOS test. The rapid IRG assay system was sensitive enough to use in the IOS test at high levels of IRG concentration, but its sensitivity needed to be improved when the IRG concentration was lower.

Key words
rapid assay of gastrin, intraoperative secretin test, curative resection of gastrinoma, selective arterial secretin injection test

Reprint requests
Yasuaki Hattori First Department of Surgery, Faculty of Medicine, Kyoto University
54 Kawarachou, Shogoin, Sakyo-ku, Kyoto, 606 JAPAN

Accepted
December 13, 1989

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