Immunohistochemical Study of Development of Colorectal Neoplasma

Osamu Mitsugi

First Department of Surgery, Yamanashi Medical College

In order to study changes in complex carbohydrates of the large intestine, I used three kinds of lectins, peanut agg lutinin (PNA), Ulex aeropaeus agglutinin-l (UEA-l), Glycine max (SBA). These lectin binding sites were examined in human normal mucosa, neoplasma, l.2-dimethyl hydrazine (DMH)) induced rat neoplasma and normal mucosa. The cell sarface staining increased in parallel with the grade of cellular dyspiasia in human PNA, UEA-1 and SBA and rat UEA-1, and SBA staining and the diffuse staining of cytoplasma increased in parallel with the grade of cellular dysplasia in PNA, UEA-I and SBA staining of human and rat neoplasma. In the distal normal mucosa of rats, PNA, and UEA-1 binding sites were found by DMH inject. These results suggest that both adenoma in the human large instestine and rat dysplasia induced by DMH are precancerous lesion and that a chemical metabolic change may occur before histological malignant transformation and that the dysplasia developing from the high risk mucosa is the origin of the cancer.

Key words
colorectal neoplasma, lectin, adenoma, dysplasia of colorectal mucosa, 1, 2-dimethylhydrazine

Reprint requests
Osamu Mitsugi First Department of Surgery, Yamanashi Medical College
1110 Shimokato, Tamaho-cho, Nakakoma-gun, Yamanashi, 409-38 JAPAN

Accepted
April 11, 1990

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