An Experimental Study for Mucosal Ischemia and Reperfusion Injuruy in the Small Intestine

Masato Takamori

Department of Surgery II, School of Medicine, Kanazawa Univeristy (Director: Prof. Itsuo Miyazaki)

We studied the protective effect of perfusion of allopurinol, an inhibitor of xanthine oxidase and suppresser of production of free radicals, on mucosal damage of the small intestine after ischemia and resumption of blood flow. Conclusions were drawn particularly from the changes in Na⁺-K⁺ ATPase actvity observed in the intestinal villi of the canine. Na⁺-K⁺ ATPase activity was significantly lower in the groups perfused and treated with or without the superoxide production inhibitor than in the control group (p<0.05). In the group given the superoxide production inhibitor in conjunction with perfusion, the reduction in Na⁺-K⁺ ATPase activity was significantly suppressed (p<0.05). The results show that Na⁺-K⁺ ATPase activity in the small intestine declines as a result of mucosal damage after ischemia and resumption of blood flow. Furthermore, the decline is caused by the involvement of free radicals, and that the administration of allopurinol as a superoxide production inhibitor is effective in protecting the mucous membrane of the small intestine after ischemia and resumption of blood flow.

Key words
small intestine, ischemia of small intestine, free radicals, allopurinol, Na⁺-K⁺ATPase

Reprint requests
Masato Takamori Department of Surgery, II, School of Medicine, Kanazawa University
13-1 Takaramachi, Kanazawa, 920 JAPAN

Accepted
May 9, 1990

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