Investigation of Hepatic Energy Metabolism in Normothermic Hepatic Ischemia -The Effect of Calmodulin Antagonist on Normal and Cirrhotic Rat Liver-

Minoru Izu, Toshiyuki Sumita, Hajime Shiga, Yoshimichi Omori, Takeshi Nagao, Hideaki Saitou, Nobuhiro Kawano, Yasuhiko Morioka

First Department of Surgery, University of Tokyo

We investigated whether the calmodulin antagonist, elziverine was effective in improving the survival rate after hepatic ischemia in normal and cirrhotic rats. We measured ATP in hepatocytes by HPLC and ³¹P-NMR to observe the effect of elziverine on the hepatic energy status during ischemia. ATP in hepatocytes of the elziverinetreated group disappeared more rapidly after hepatic ischemia than in those of the untreated group. Therefore, the potential of the hepatocytes was maintained, and the survival rate for normal and cirrhotic rats after hepatic ischemia was improved. Lactic acid in hepatic tissue increased before hepatic ischemia in the elziverine treated group, and elziverine inhibited elevation of phosphomonoester in hepatocytes during ischemia. It is possibly that elziverine promoted the system of glycolysis during hepatic ischemia. Elziverine was effective in improving the survival rate after hepatic ischemia, through promoting glycolysis and resolution of ATP in hepatocytes after hepatic ischemia.

Key words
hepatic ischemia, ³¹P-NMR, calmodulin-antagonist, cirrhosis, energy status

Reprint requests
Minoru Izu First Department of Surgery, University of Tokyo
7-3-1 Hongo, Bunkyou-ku, Tokyo, 113 JAPAN

Accepted
January 16, 1991

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