A Study on Intraoperative Bacterial Culture of Bile Juice from Gall Bladder and Postoperative Bacterial Culture from T-tube

Hiroyuki Naitoh, Yoshimasa Kurumi, Junsuke Shibata, Takanobu Hase, Kazuya Tsuji, Yoshihiro Endo, Tooru Tani, Hiroki Tamura, Masashi Kodama

First Department of Surgery, Shiga University of Medical Science

We analyzed cultures of bile juice taken from the gall bladder of 158 patients with cholelithiasis during surgery, and from the T-tube of 48 patients after surgery. The rate of isoration of Escherichia coli (18 patients), Klebsiella (12 patients) and Enterococcus faecalis (11 patients) from bile of the gall bladder during the operation were high. The rate of isolation of bacteria from bile taken during surgery was high in patients with a past history of some symptoms of cholelithiasis, aged over 60 years, and having an abnormality of liver function or having fever or leukocytosis during the week before the operation. With each day, the rates of isolation of E. coli and Klebsiella decreased but that of Enterococcus faecalis increased in cultures of bile from the T-tubes. E. coli (94.9%) and Klebsiella (81.9%) showed high sensitivity to antibiotics, but Enterococcus faecalis (53.3%) showed low sensitivity. That was one of the reasons the bacteria from the T-tubes changed. We experienced 18 cases of infectious complication after the operations of 158 patients, and the bacteria were the same as those from the bile juice taken from the gall bladder in 13 cases.

Key words
cholelithiasis, intraoperative culture of bile juice of gall bladder, postoperative culture of bile juice of cheledocus from T-tube, enterococcus faecalis, infectious complication after operation of cholelithiasis

Reprint requests
Hiroyuki Naitoh The First Department of Surgery, The Shiga University of Medical Science
Seta Tsukiwa-cho, Otsu, 520-21 JAPAN

Accepted
May 8, 1991

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