Characterization and Metastatic Potential of a Mouse Adenocarcinoma Cell Line XK-4 which arose in Nude Mouse during the Transplantation of Human Colon Cancer Cell Line KUM·RK-4N

Toshiyuki Adachi, Haruhiko Inufusa, Nabuhira Mori, Satoshi Hara, Noriyuki Sagara, Masayuki Yasutomi

First Department of Surgery, Kinki University School of Medicine

A mouse adenocarcinoma cell line that arose spontaneously in a nude mouse was established during the transplantation of a human colon cancer tumor line into the subcutaneous tissue of the nude mouse. Human colon cancer tumor line KUM·RK-4N was established in a nude mouse serially transplanted with human colon adenocarcinoma in October 1985. The cell line which we established was primary cultured from the KUM·RK-4N tumor in February 1990. Metastatic nodules were clearly produced in lung and liver after 3∼4 weeks when the cells were injected into the tail vein and anterior mesenteric vein. The histological pattern of the subcutaneous tumor produced from the established cell line was a moderately differentiated adenocarcinoma similar to the KUM·RK-4N cell line. Chromosomal analysis of this cell line revealed only murine chromosomes and isozymes analysis showed a murine pattern. Based on these results this cell line was named mouse adenocarcinoma cell line XK-4. Transplantation of XK-4 cells into thymus-intact littermates was rejected, suggesting that XK-4 cancer grew spontaneously in the nude mouse. In this study, a new adenocarcinoma cell line XK-4 was established and the metastatic potential to lung and liver was characterized.

Key words
nude mouse, spontaneous tumor in nude mouse, liver metastatic model, lung metastatic model

Reprint requests
Toshiyuki Adachi First Department of Surgery, Kinki University School of Medicine
377-2 Ohno-Higashi, Osaka-Sayama, 589 JAPAN

Accepted
September 4, 1991

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