Comparison of DNA Content between Primary Tumor and Metastatic Lymph Node in Colorectal Carcinoma

Shinji Ushida, Kazuyoshi Kurooka, Sadao Funai, Hiromi Yamada, Yoshinori Fujii, Yukio Imanishi, Taiji Matsuda, Katsuhisa Shinodo, Masayuki Yasutomi

First Department of Surgery, Kinki University School of Medicine

Nuclear DNA content was determined in 143 cases of colorectal carcinoma by using flow cytometry, and relationship between DNA index (DI) and lymph node metastasis was studied. Moreover, we determined nuclear DNA content in 21 cases of metastatic lymph nodes and compared them with the primary tumor. With regard to the DNA ploidy pattern, there were 50 cases of DNA diploidy and 93 cases of DNA aneuploidy, so that the respective rates observed in lymph node metastasis were 30.0% (15/50) and 62.4% (58/93). Thus DNA aneuploidy was observed at a significantly higher rate. The lymph node metastatic rate tended to be DI=1.3 (75%), 1.5 (71.4%), 1.6 (100%), and 1.7 (70.0%). In addition, in 21 cases where the nuclear DNA content was determined in the metastatic lymph nodes, the DI between the primary tumor and the metastatic lymph node agreed in all cases.

Key words
flow cytometry, nuclear DNA content, lymph node metastasis of colorectal cancer

Reprint requests
Shinji Ushida First Department of Surgery, Kinki University School of Medicine
377-2 Ohno-higashi, Osaka Sayama, 589 JAPAN

Accepted
October 9, 1991

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