Effects of Prostaglanding-E₁ Derivative on Regenerating Liver after Partial Hepatectomy in Rats with Obstructive Jaundice

Fumio Kimura, Masaru Miyazaki, Ikuo Udagawa, Hisao Koshikawa, Takashi Kaiho, Jun Matsumoto, Toshio Isono, Hiroyuki Suzuki, Katsuji Okui

The First Department of Surgery, Chiba University School of Medicine

We examined the relationship between DNA synthesis and protein synthesis of regenerating liver after partial hepatectomy in rats with obstructive jaundice and determined the effects of a PG-E₁ derivative on liver regeneration under that condition. The bile duct of rats were ligated and 5 or 14 days later partial hepatectomy and choledocho-duodenostomy were performed to relieve the obstruction. Twenty-four hours later, (³H)-thymidine incorporation by hepatic DNA was significantly lower in the jaundiced rats than in the controls that received partial hepatectomy alone. However, (¹⁴C)-leucine incorporation by hepatic proteins was markedly increased in the jaundiced rats. Hepatic protein synthesis was also increased 48 hours after partial hepatectomy in the normal rats, but there were no significant changes in the jaundiced rats. PG enhanced the DNA synthesis and the hepatic protein synthesis of the regenerating liver inhibited by obstructive jaundice. We concluded that the preceding energy-requiring responses in obstructive jaundiced liver, such as enhancement of hepatic protein synthesis, resulted in the reduction of hepatic DNA synthesis and in the lack of an additional increase in hepatic protein synthesis as responses to the further insult of partial hepatectomy.

Key words
obstructive jaundice, liver regeneration after partial hepatectomy, hepatic protein synthesis, DNA synthesis in regenerating liver, prostaglandin-E₁ derivative

Reprint requests
Fumio Kimura The First Department of Surgery, Chiba University School of Medicine
1-8-1 Inohana, Chiba City, 280 JAPAN

Accepted
November 20, 1991

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