The Intestinal Tissue Damages and the Changes of Reactive Oxygen Species due to Ischemia, Congestion and Reperfusion

Yoshihiro Moriwaki, Hiroshi Katamura, Yasushi Ichikawa, Toshiroh Yamamoto, Mitsugi Sugiyama*

Second Department of Surgery and The Department of Critical Care and Emergency*, Yokohama City University, School of Medicine

Tissue damage after declamping and the role of reactive oxygen species in this damage was compared in an ischemia group in which the intestinal segment and the vessels supplying this segment were clamped for 10 and 30 minutes, and a congestion group in which the segment and its draining veins were clamped for 10 and 30 minutes (n=80). The effect of radical scavangers on the damage due to ischemia, congestion and reperfusion was also investigated (n=16). The tissue damage was severer in the congestion group than in the ischemia group with the same clamping time, and when the time of clamping was longer, the damage after declamping was severer. The level of lipid peroxide (LPO) in the involved bowel and chemiluminescense (CL) of the vein draining from the involved bowel were also higher in the congestion group when the clampling time was the same. But in the congestion group, the level of LPO and CL was higher after the release of 10 minute's clamping than after 30 minute's clamping, in which the tissue damage during congestion was too severe to result in tissue necrosis. The increments in LPO and CL were suppressed by treatment with radical scavengers.

Key words
ischemia-reperfusion of small intestine, congestion-reperfusion of small intestine, oxygen derived free radicals, liqid peroxidation

Reprint requests
Yoshihiro Moriwaki Second Department of Surgery, Yokohama City University School of Medicine
3-46 Urafune-chou, Minami-ku, Yokohama, 232 JAPAN

Accepted
September 9, 1992

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