Study of the Gastric Mucosal Cytoprotection in Burn Stress with Special Reference to Gastric Mucosal Gylcoprotein

Mizuhiro Mogi

First Department of Surgery, Kyorin University, School of Medicine

[Purpose] We used lectin to investigate the effect of gastric acid secretion inhibitors based on changes in gastric mucosal glycoproteins after experimental burn injury, in comparison with a control. [method] Male Wistar rats were subjected to burn injury and examined using PNA lectin. [Results] PNA lectin binding sites which were primarily localized to the generative cell zone in the normal control rats, appeared to extend from the upper layer to the intermediate or basal layer of the gastric mucosa in accordance with the effects based on the dose of H₂ receptor antagonist, and used Ranitidine 20 mg/kg. After the burn injury the same tendencies were noted in the vagotomy group and those treated with the H₂ receptor antagonist group. Electron microscopically, most of the binding sites were detected in the parietal cells, particularly along microvilli in the intracellular secretory canaliculi. The tubulovesicular system in the parietal cells became stainable with PNA lectin in the stress'loaded rats. Similar electron microscopic findings were obtained even in vagotomy group and with the H₂ receptor antagonist group. In this study, there were no differences according to the dose of Omeprazole among the groups receiving the drugs. Although similar tendencies were observed in the Omeprazole groups after burn injury, the PNA lectin bindig sites were inhibited. [Conclusion] Changes in the lectin binding pattern made it possible to postulate a local cytoprotective reaction against stress. It also appeared that Omeprazol inhibits this lectin binding pattern, and based on the electron microscopic findings, that parietal cells partcipate in the cytoprotective reaction. H₂ receptor antagonist and vagotomy had pharmacological effects on a increase of PNA lectin binding sites.

Key words
PNA lectin binding sites, parietal cell, burn stress ulcer in rat, cytoprotection of gastric mucosa

Reprint requests
Mizuhiro Mogi First Department of Surgery, Kyorin University, School of Medicine
6-20-2 Shinkawa, Mitaka, 180 JAPAN

Accepted
October 13, 1993

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