Influence of Obstructive Jaundice on Experimental Acute Gastic Ulcer with Electronmicroscopic Study of Histometric Mitochondrial Structures of Gastic Mucosal Parietal Cells in Rats

Yoshio Matsuda, Iwao Sasaki, Yasuhiko Kamiyama, Yuji Funayama, Hiroo Naito, Seiki Matsuno

The First Department of Surgery, Tohoku University School of Medicine

To determine the effect of obstructive jaundice on electronmicroscopic changes of mitochondrial structures of parietal cells during water-immersion restraint stress, male Sprague-Dawley rats weighing about 200 g were used. Experiment 1, control (sham operation), 2-, 4-, 6-, and 8-week jaundiced (bile duct ligation) rats and biliary drained rats were used to study the effect of duration of jaundice. Experiment 2.2-week jaundiced rats and biliary drained rats were used to study the effect of the stress on jaundice and biliary drainage. The number of crystae was significantly higher in the 2-week jaundiced group than in the control group. Fusion and deformity of mitochondria were seen in the 4-, 6-, and 8-week jaundiced groups. In the 2-week jaundiced group, the mitochondria were restored to normal by biliary drainage. Mitochondrial damage during the stress in the 2-week jaundiced group was more severe than in the control group. These results indicate that an acute gastric mucosal lesion can easily occur in obstructive jaundice owing to severe disturbance of energy metabolism, which is demonstrated by histometry of the mitochondrial structure in parietal cells. Biliary drainage will protect this ulceration by reducing mitochondrial damage.

Key words
acute gastric ulcer, obstructive jaundice, ultrastructure of parietal cell, water-immersion restraint stress, effect of biliary drainage

Reprint requests
Yoshio Matsuda The First Department of Surgery, Tohoku University School of Medicine
1-1 Seiryocho, Aobaku, Sendai, 980 JAPAN

Accepted
December 8, 1993

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