A Cytogenetic Analysis of Human Esophageal Carcinomas in term with Their Prognosis Using int-2, hst-1 and RB Gene Probes

Yasuyuki Kureyama, Makoto Kataoka, Yoshiyuki Kuwabara, Hiroshi Iwata, Taketoshi Kashima, Noriyuki Shinoda, Atsushi Sato, Koji Hattori, Koichiro Nakano, Naoki Nishiwaki, Akira Masaoka

Second Department of Surgery, Nagoya City University Medical School

We analyzed the correlation between clinicopathological factors and int-2 and hst-1 gene amplification and RB-LOH in 17 patients with esophageal carcinoma who underwent surgery from 1989 to 1991. The rates of int-2 and hst-1 gene amplification were 59% and 53%, respectively. Int-2 and hst-l amplification were not correlated with histological type or clinical stage, but were significantly correlated with sex, distant metastasis, venous invasion and lymphatic invasion. Patients with amplification of these genes especially with more than 4-fold amplification had poorer prognosis due to distant metastasis than those without amplification. The incidence of RB-LOH was 56%. RB-LOH was correlated with lymphnode metastasis, venous invasion and clinical stage, although RB-LOH showed no tendency in the analysis of survival. These results revealed that int-2 and hst-1 gene amplification is a useful biological indicator that is not related to histological malignancy.

Key words
int-2, hst-1 oncogene, RB tumor suppressor gene, esophageal carcinoma, prognostic factor

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Yasuyuki Kureyama The Second Department of Surgery, Nagoya City University Medical School
1 Kawasumi, Mizuhocho, Mizuho-ku, Nagoya City, 467 JAPAN

Accepted
March 2, 1994

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