A Basic Study of Photodynamic Therapy of Experimental Hepatoma with PH-1126

Hirohisa Kajiwara

Third Department of Surgery, Toho University

After VX2 hepatoma models were prepared using Japanese white rabbits, we carried out fundamental studies on the photodynamic therapy with PH-1126, a photosensitizer, and krypton laser in the treatment of hepatoma; namely, accumulation of PH-1126 in livers of these models was measured 48 hours after administration at a dosage of 3 mg/kg, using 2 methods, endoscopic photometer and macroscopic observation with a surgical microscope. More, we also determined the therapeutic effects of the photodynamic therapy with a krypton laser at 80 J/cm² 48 hours after administration of PH-1126. The tumor models were as follows: Control: rabbit hepatoma treated by intratumoral three-point irradiation without PH-1126. Group 1: non-contact surface irradiation method (used with fiber of the forward irradiation type). Group 2: intratumoral one-point irradiation method. Group 3: intratumoral three-point irradiation method. (Omnidirectional fiber was used in Groups 2 and 3) Anti-tumor effects were examined as to (1) inhibitory effect on tumor growth and (2) necrotizing rate. It was found that use of the 3-point irradiation method gave significantly better therapeutic results compared with the other three methods. The survival time was also significantly prolonged in the 3-point irradiation group. From these results, it was confirmed that the photodynamic therapy with PH-1126 is useful as a new multidisciplinary therapy for hepatoma.

Key words
photodynamic therapy, PH-1126, VX2-tumor

Reprint requests
Hirosa Kajiwara Third Department of Surgery, Toho University, School of Medicine
2-17-6 Ohashi Meguro-ku, Tokyo, 153 JAPAN

Accepted
February 8, 1995

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