Immunohistochemical Analysis of nm23 Gene Product/NDP Kinase Expression in Bile Duct Carcinoma -Lack of Prognostic Value-

Ryouko Sasaki, Senji Kanno, Takayuki Suto, Hiroyuki Nitta, Masahiko Murakami, Yoshiro Hayakawa, Yutaka Shimada, Hidenobu Kawamura, Yoshiyuki Tamasawa, Kazuyoshi Saito

Department of Surgery 1, Iwate Medical University School of Medicine

The expression of nm23 gene products, that is, NDP kinase were investigated immunohistochemically in 54 resected cases using monoclonal antibodies for two types of isoform. The expression rate of nm23-H1 was 79.6% and that of nm23-H2 was 85.2%. The expression rate of nm23-H1 was significantly lower in the cases of duodenal invasion, but there were not significant relation between the expression of nm23 and depth of invasion lymph node metastasis and pancreatic invasion. There was no significant correlation between the expression of nm23-H1 and nm23-H2, and prognosis (p=0.79 and p=6.63, respectively). The expression rate of nm23 was not significantly different among patients with no recurrence over 3 years, those with no recurrence over 5 years and those who had liver metastasis after surgery. These results suggest that there is no significant correlation between the expression of nm23 and lymph node metastasis, prognosis and liver metastasis, and nm23 may lack value as an indicator of prognosis or metastasis in bile duct carcinoma

Key words
bile duct carcinoma, nm23, nucleoside diphosphate kinase, immunohistochemistry

Reprint requests
Ryouko Sasaki Department of Surgery 1, School of Medicine, Iwate Medical University
19-1 Uchimaru, Morioka, 020 JAPAN

Accepted
March 8, 1995

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