Experimental Studies for the Mechanism of Intestinal Ischemic-Reperfusion Injury

Masami Ikeda, Sumito Takagi, Kuniyoshi Yamagata, Akio Hara, Hiroshige Yoshida, Kazuo Ueda, Tadaaki Shiba, Setsuo Takeuchi, Shiro Tsujimoto*

Second Department of Surgery, Toho University School of Medicine
Department of Pathology, Omori Hospital*

Recently, active oxygen has been identified as a factor in intestinal ischemic-reperfusion injury. The authors have reported on chemical mediators such as blood-platelet activating factor (PAF) and leucotrienes (LTB4·C4) as important factors. These together with active oxygen cause mucous disorders after reperfusion, but, to investigate the injury as it appears during ischemia, we prepared intestinal ischemic-reperfusing dogs. An endoscope was used to observe the mucous membrane during ischemia over a period of time, and PAF and LTB4·C4 were measured. During intestinal ischemia, PAF increased in the tissue, and after reperfusion. LTB4·C4 appeared. Endoscopic observation revealed that intestinal ischemic-reperfusion injury showed a state of preparation with ischemia that became more marked with reperfusion. In contrast, during ischemic-reperfusion under the administration of highly concentrated oxygen, there was no increase in PAF during ischemia, and the mucous problem was slight. We emphasize the importance of the role played by PAF during the entire course of ischemic-reperfusion.

Key words
intestinal ischemic-reperfusion injury, platelet activating factor, leukotrienes, dogs experi-mental study

Reprint requests
Masami Ikeda Second Department of Surgery, Toho University School of Medicine
6-11-1 Omorinishi, Ota-ku, Tokyo, 143 JAPAN

Accepted
September 13, 1995

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