Strategy for the Possible Microscopic Liver Metastasis of Colorectal Cancer -For the Future Gene Therapy for Colorectal Cancer-

Takashi Ishikawa, Yasushi Ichikawa, Nobuyoshi Momiyama, Hideyuki Ike, Shigeki Yamaguchi, Hidenobu Masui, Masao Nanko, Hiroyuki Yamaoka, Masato Mitsuhashi¹⁾, Tatsuo Akitaya²⁾, Hiroshi Shimada

Second Department of Surgery, Yokohama City University, School of Medicine
¹⁾Hitachi Chemical Research Center, Irvine, CA, USA
²⁾Hitachi Chemical Company

Messenger RNA expression of matrix metalloproteinase (MMP) 2,7,9 was examined in surgical specimens of colorectal cancers. Only MMP-7, matrilysin, mRNA was limitedly expressed in cancer, but not in surrounding tissue. Its expression was associated with progression of colorectal tumors with the maximum in liver metastatic tumors. Antisense oligonucleotides for matrilysin suppressed matrilysin mRNA expression in a matrilysin-producing rectal cancer cell line, CaR-l, resulting in inhibition of cell proliferation and the invasiveness of this cell line dose dependently. We conclude that matrilysin is a promising candidate as a new clinical diagnostic and prognostic marker of colorectal cancers as well as antisense oligonucleotide therapy.

Key words
matrilysin, antisense oligonucleotide, colorectal cancer

Reprint requests
Takashi Ishikawa Second Department of Surgery, Yokohama City University, School of Medicine
3-9 Fukuura, Kanazawa-ku, Yokohama, 236

Accepted
November 15, 1995

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