Organ Xenotransplantation with Gene Enginerring

Shuji Hayashi, Takaharu Nagasaka, Akiko Katayama, Masataka Negita, Chihiro Koike, Itsuo Yokoyama, Hiroshi Takagi

Department of Surgery II, Nagoya University School of Medicine

Organ xenotransplantation is an attractive method to compensate for the cadaveric donor shortage. However, hyperacute rejection is a great barrier to successful organ xenotransplantation. In this study, the authors examined the effect of in vitro gene engineering with regulator of complement activation (RCA) molecules such as DAF and HRF20, and the possibility of applying in vivo gene enginerring to the xenogeneic liver and kidney using an adenoviral vector. It was concluded that complement activation is inhibited by the gene engineering with RCA molecules against the xenogeneic cells, and that an adenoviral vector is a useful method for in vivo gene engineering to the xenogenic organs.

Key words
xenotransplantation, gene engineering, complement activation

Reprint requests
Shuji Hayashi Department of Surgery II, Nagoya University School of Medicine
65 Tsurumai-cho, Showa-ku, Nagoya, 466 JAPAN

Accepted
December 6, 1995

To read the PDF file you will need Abobe Reader installed on your computer.