Endothelial Cell Damage during Liver Ischemia and Protection using Hepatic Perfusion with Nafamostat Mesilate in Rats

Norio Yasuda, Masaru Miyazaki, Katsuhiro Iinuma, Masayuki Ohtsuka, Nobuyuki Nakajima

First Department of Surgery, School of Medicine, Chiba University

In situ isolated liver perfusion was done during liver ischemia for 60 minutes in the rats. The effect of various perfusates and nafamostat mesilate (NM) on hepatic ischemic damage, especially on sinus endothelial cell damage, was assessed. Five different perfusate groups were assessed: group A (lactated Ringer [LR] at 25°C), group B (LR at 4°C), group C (LR with NM of l0 µg/ml at 25°C), group D (LR with NM of l0 µg/ml at 4°C), group E (University of Wisconsin solution at 4°C). The addition of NM in liver perfusion markedly inhibited the increases in GOT, TXB₂, 6 keto PGF_1α in the perfusate. The swelling of hepatocytes was significantly suppressed in group E. However, the sinus endothelial cell damage was most remarkably reduced by addition of NM (group C and D). Hepatic perfusion with NM might achieve its hepatoprotective effect by reducing sinus endothelial cell damage during liver ishcemia.

Key words
hepatic perfusion, Nafamostat mesilate, hepatic ischemia, sinus endothelial cell

Reprint requests
Masaru Miyazaki The First Department of Surgery, School of Medicine, Chiba University
1-8-1 Inohana, Chuoku, Chiba, 260 JAPAN

Accepted
March 6, 1996

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