Studies on the Lymphocyte Subsets of the Patients with Gastroenterological Cancer by Use of Three Color Flow Cytometry

Hidenori Sumida, Makoto Kataoka, Yoshiyuki Kuwabara, Hiroyuki Kawamura, Masami Mitani, Atsushi Satou, Kouji Hattori, Naoki Nishiwaki, Kouichirou Nakano, Masahiro Kimura, Kiyoshi Narita, Hiroshi Koyama, Takehiro Katou, Akira Masaoka

The Second Department of Surgery, Nagoya City University Medical School

In order to assess the immune activity in patients with cancer patient, the lymphocyte subsets of 73 gastroenterological cancer (45 gastric cancer, 16 colon cancer, 6 esophageal cancer, 3 hepatoma, 3 pancreatic cancer) patients and 17 non-cancer patients were analyzed with monoclonal antibodies. In T cells, CD3 (+) HLA-DR (+) (activated T) cells and CD8 (+) CDl1b (+) (suppressor T) cells tended to increase in cancer patients and with advance in stage. Natural killer (NK) cells increased in cancer patients and in the subsets of NK cells, CD57 (+) CD16 (-) [minimal lytic activity] cells and CD57 (+) CD16 (+) [moderate lytic activity] cells tended to increase with advance in stage. Lymphokine activated killer (LAK) cells and an increase in CD3 (+) CD16 (-) CD56 (+) (T-LAK) cells tended to increase with advance in stage.

Key words
three color flow cytometry, lymphocyte subsets, gastroenterological cancer

Reprint requests
Hidenori Sumida The Second Department of Surgery, Nagoya City University Medical School
1-1 Aza-Kawasumi Kawasumi-cho, Mizuho-ku, Nagoya City, 467 JAPAN

Accepted
February 14, 1996

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