A Study of Novel Kits for Peritoneal Irrigation Cytology by Release of Carcinoem Bryonic Antigen for Incerase of its Sensitivity

Takashi Suhara, Kyoshi Tsuji, Motohisa Kato, Katsuyuki Kunieda, Takao Umemoto, Kiichi Miya, Shigetoyo Saji

Second Department of Suugery, Gifu University School of Medicine

CEA antigen is a membrane-bound phosphatidylinositol anchor, and is released from cancer cells with phosphatidylinositol phospolipase c (PIPLC). By useing of this phenomenon, we produced a novel kit for peritoneal irrigation cytology. The peritoneal sediment obtained from peritoneal irrigation and filtered through a glass microfibre filter was fixed on a plate coated with anti-CEA antibody and 0.05 U of PIPLC was added. The reaction was considered positive if there was a color difference between the plates with PIPLC and without it after incubation for 60 minutes and colored by the sandwich method (PIPLC-kit). The PIPLC-kit and conventional irrigation cytology (cytology) were compared in 63 gastric cancer patients. All of the 16 patients showing a positive reaction by cytology were also all positive by the PIPLC-kit, while the 47 patients negative by cytology 5 patients were positive by the PIPLC-kit. Moreover, of the 52 patients without macroscopic peritoneal metastases 5 were positive by cytology and 10 by the PIPLC-kit. The above results suggest that the PIPLC-kit is a useful and simple assay system for the detection of early peritoneal dissemination.

Key words
peritoneal dissemination, irrigation cytology, CEA, phosphatidylinositol phospholipase C, assay system

Reprint requests
Takashi Suhara Second Department of Surgery, Gifu University School of Medicine
40 Tsukasamachi, Gifu, 500-8705 JAPAN

Accepted
September 16, 1998

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