Suppression of Bacterial Translocation by L-Glutamine and Polydextrose in Rats, Particularly Evaluated from Changes of Nitric Oxide

Hidemi Yamaguchi

Second Department of Surgery, Dokkyo University School of Medicine

Using the jejunoileal bypass (JIB)-induced bacterial translocation (BT) model in rats, the effect of L-glutamine and polydextrose was investigated for prevention of BT with changes in local nitric oxide (NO) and inducible NO synthase mRNA. The rats were divided into 3 groups according to the material administered intraluminally: Group C (control) received infusion of only 5% glucose. Group G received infusion of 5% glucose + L-glutamine. Group P received 5% glucose + polydextrose. Seven days after JIB, increases in NOx, GOT, GPT levels in the portal vein were demonstrated in group C. In group G, these three levels statistically showed a tendency of decrease as compared with those in group C. In group P, the level of NOx was statistically suppressed significantly. Although no statistically significant difference was found in the levels of serum GOT and GPT, there was a tendency of decrease. Translocation of Escherichia coli to mesentric lymph nodes and atrophy of the intestinal mucosa revealed a tendency of decrease in groups G and P. As a conclusion, intraluminal administration of L-glutamine and polydextrose seems to suppress the appearance of local NO and BT.

Key words
bacterial translocation, nitric oxide, glutamine, polydextrose

Reprint requests
Hidemi Yamaguchi Second Department of Surgery, Dokkyo University School of Medicine 880 Kita-kobayashi, Mibu, Shimotsuga, 321-0293 JAPAN

Accepted
October 14, 1998

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