The Expression of Cell Cycle Regulators and Malignant Potential of Gastric Carcinomas -especially, Gastric Carcinoma with Submucosal (sm) Invasion-

Yoshihiro Kagawa, Kazuhiro Yoshida, Naoki Nishimoto, Yasuo Yano, Toshihiro Hirai and Tetsuya Toge

Department of Surgical Oncology, Research Institute for Radiation Biology
and Medicine, Hiroshima University

In this study, we analysed the relationship between the expression of cell cycle regulators and clinicopathological parameters of gastric carciomas. We examined the expression of p27^Kip1 (p27), cyclin E, p53 and p21^WAF1 (p21) in gastric carciomas, especialy in order to predict lymph node metastasis of gastric carciomas with sm invasion. One hundred and seventy eight gastric adenocarcinomas were examined and Immunohistochemical staining was performed with p27, cyclin E monoclonal antibodies. Sixty nine gastric carciomas with sm invasion were also stained with p53, p21 monoclonal antibodies. The relationship between the grade of expression and clinicopathological parameters was examined. Low grade expression of p27 was detected in undifferentiated type, the cases with lymph node metastasis, and advanced stage. Moreover, low grade expression of p27 and high grade expression of cyclin E had a strong correlation with lymph node metastasis. The prognosis of these patients had no correlation with grade of expression of p27 or cyclin E. On the other hand, in gastric carciomas with sm invasion, high grade expression of p27 correlated with differentiation and lymph node metastasis. High grade expression of cyclin E and p21 also showed correlation with lymph node metastasis. Further analysis should be performed in order to predict lymph node metastasis of gastric carcinomas with sm invasion.

Key words
gastric carcioma, submucosal invasion, p27^Kip1, cell cycle regulator, lymph node metastasis

Reprint requests
Yoshihiro Kagawa Department of Surgical Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi Minami-Ku, Hiroshima, 734-8553 JAPAN

Accepted
January 26, 2000

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