ORIGINAL ARTICLE
The Influence of Liver Ischemia-Reperfusion on the Development of Hepatic Metastases in Rat
Ichiro Sonoda, Tastuya Aoki and Tatsuto Ashizawa
Department of Surgery, Tokyo Medical University (Director: Prof. Yasuhisa Koyanagi)
We evaluated the hypothesis that hepatic ischemia-reperfusion injury contributes to liver metastasis, and determine whether expression of intercellular adhesion molecule 1 (ICAM-1) on the sinusoidal wall increased under such conditions. Subjects were divided into 5 groups, A to E. In group A involving 30 minutes of right-lobe ischemia by clamping, ICAM-1 expression was greater in the ischemic lobe than in the nonischemic lobe. In group B involving splenic ACL-15 cells injection, 5 of 12 subjects developed liver metastasis, but no statistically significant difference was seen between the right and median and left lobes in the number of tumor foci. In group C involving 30 minutes of right-lobe ischemia plus splenic injection and group D involving 30 minutes median- and left-lobe ischemia plus splenic injection, ICAM-1 expression of in the ischemic lobe was significantly greater than that in the nonischemic lobe, with the number of tumor foci in the ischemic lobe significantly higher than that in the nonischemic lobe (group C: p<0.05; group D: p<0.05 1,-tail P). In group E involving 30 minutes of right-lobe ischemia plus splenic injection 5 minutes after anti-ICAM-1 monclonal antibody given through the superior mesenteric vein, no statistically significant difference was seen between the ischemic and nonischemic lobes in the number of tumor foci, suggesting that hepatic ischemia-reperfusion injury accelerates liver metastasis and that ICAM-1 may be associated with the development of hematogenous hepatic metastasis.
Key words
ischemia-reperfusion injury, intercellular adhesion molecule-1 (ICAM-1), hematogenous hepatic metastasis
Jpn J Gastroenterol Surg 34: 177-188, 2001
Reprint requests
Ichiro Sonoda Department of Surgery, Tokyo Medical University 6-7-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo, 160-0023 JAPAN
Accepted
December 19, 2000
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