The Protective Effect of Ischemic Preconditioning on Ischemia-reperfusion injury of the Liver: Relationship between the Glycogen Consumption in the Liver Tissue and Ischemic Preconditioning

Kiyoshige Hamada

Second Department of Surgery, Dokkyo University School of Medicine

In a study of the effects of ischemic preconditioning (IP) on hepatic ischemic-reperfusion injury, we divided Wistar rats into 3 groups: group A, 10 min clamping followed by 5 min release., group B, 30 min clamping followed by 5 min release., and group C, no IP. Following IP, we clamped the hepatoduodenal ligament for 60 min and then released. We then assessed changes of serum levels of asparate aminotransferase (AST), alanine aminotransferase (ALT), uric acid, energy charge, glycogen, and lactate in liver tissue, together with blood flow and histopathological findings in liver tissue. In group A, AST, ALT and lactate in liver tissue 60 min after declamping were significantly lower than in group B and C. The glycogen was adequately consumed each measurement time, and blood flow in tissue was well preserved in group A. Histologically, damage to hepatocytes was minimal and glycogen granules decreased as time passed. IP with 10 min clamping followed by 5 min release effectively decreased damage of ischemic-reperfusion injury and IP effects may be based on the glycogen consumption in liver tissue.

Key words
ischemic preconditioning, glycogen consumption in the liver tissue, uric acid, lactate, pringle maneuver

Reprint requests
Kiyoshige Hamada Second Depertment of Surgery, Dokkyo University School of Medicine 880 Kita-kobayashi, Mibu, Shimotuga, 321-0293 JAPAN

Accepted
December 19, 2000

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