Possibility of Pre-adjusting Exogenous Liquid Fibrin Glue Prior to the Surgical Operation

Masanori Suzuki, Tohru Onogawa, Michiaki Unno, Kojin Endo, Yu Katayose, Heigo Takeuchi, Masaya Oikawa, Noriko Kondo and Seiki Matsuno

Department of Surgery, Division of Gastroenterological Surgery, Tohoku University Graduate School of Medicine

Background: Liquid type fibrin glue should, in principle, be prepared immediately before use. We studied the feasiblity of dissolving fibrin glue in advance of surgery. Materials and Methods: Solutions A and B were prepared using Beriplast^® (Aventis Pharma Co. Ltd., Tokyo, Japan). The viscosity of solitions A and B was measured throughout the time course; tensile strength was measured using the swine skin section method, and the potency of fibrinogen, thrombin, and factor XIII after preparation were also examined; and bacteria reproduction in both solutions was studied under the following 3 conditions: at room temperature (20 to 25'C), preserved in a cold place (6'C), and at 37'C. Results: (1) The tensile strength was maintained for10days under all of the above conditions, without reduction. (2) The viscosity of solution A did not change markedly immediately after dissolution to 3 days after dissolution at any temperature. The viscosity of solution B did not change immediately after dissolution and even10days after dissolution. (3) Time-course changes in fibrinogen and factor XIII activity were mild, whereas thrombin activity decreased to 48.9∼63% at 3 days in all samples, and activity continued to decrease gradually. (4) Tests conducted immediately and10days after preparation demonstrated the sterility of both solutions. Conclusion: It is feasible to prepare fibrin glue in advance of use, given its effectiveness as a biological adhesive.

Key words
fibrin glue, biological adhesives

Reprint requests
Masanori Suzuki Department of Surgery, Division of Gastroenterological Surgery Tohoku University Graduate School of Medicine 1-1 Seiryomachi, Aobaku, Sendai 980-8574 JAPAN

Accepted
September 19, 2001

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